TY - JOUR
T1 - A multicatalytical protease complex from pituitary that forms enkephalin and enkephalin containing peptides
AU - Orlowski, Narian
AU - Wilk, Sherwin
PY - 1981/8/14
Y1 - 1981/8/14
N2 - The pituitary contains a high molecular weight (M.W.∼700,000) neutral endopeptidase complex that behaves in solution as a single protein, but on polyacrylamide gel electrophoresis under dissociating conditions shows the presence of five components with molecular weights of 24,000 to 28,000. The complex exhibits trypsinlike, chymotrypsinlike and peptidylglutamyl-peptide bond hydrolysing activities. Experiments indicate that each of these activities is associated with a separate component of the complex. The chymotrypsinlike and trypsinlike activities efficiently generate Leu-enkephalin, Leu-enkephalin-Arg6 and Leu-enkephalin-Arg6-Phe7 from a single synthetic precursor. Selective inhibition of the chymotrypsinlike activity enhances the trypsinlike activity and alters the relative proportion of opioid peptides formed.
AB - The pituitary contains a high molecular weight (M.W.∼700,000) neutral endopeptidase complex that behaves in solution as a single protein, but on polyacrylamide gel electrophoresis under dissociating conditions shows the presence of five components with molecular weights of 24,000 to 28,000. The complex exhibits trypsinlike, chymotrypsinlike and peptidylglutamyl-peptide bond hydrolysing activities. Experiments indicate that each of these activities is associated with a separate component of the complex. The chymotrypsinlike and trypsinlike activities efficiently generate Leu-enkephalin, Leu-enkephalin-Arg6 and Leu-enkephalin-Arg6-Phe7 from a single synthetic precursor. Selective inhibition of the chymotrypsinlike activity enhances the trypsinlike activity and alters the relative proportion of opioid peptides formed.
UR - http://www.scopus.com/inward/record.url?scp=0019731397&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(81)91823-4
DO - 10.1016/0006-291X(81)91823-4
M3 - Article
C2 - 7030330
AN - SCOPUS:0019731397
SN - 0006-291X
VL - 101
SP - 814
EP - 822
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -