A modified technique for producing ascites experimentally

The investigation of the hemodynamic and metabolic aspects of portal hypertension and ascites, and the recent introduction of valves for draining ascites, require an experimental animal model of this condition. Several methods have been described, which involve complicated surgical techniques or are associated with a high rate of morbidity or mortality. Furthermore, they usually require a laparotomy, often resulting in adhesion and ascites that contains blood and fibrin. This may interfere with the experiments for which the ascites has been produced. Our technique of progressive constriction of the thoracic inferior vena cava in dogs is a simple surgical procedure. It avoids laparotomy, has a very low mortality rate, and results in clear, massive and long-standing ascites.