TY - JOUR
T1 - A model of immunologic lung injury induced by trimellitic anhydride inhalation
T2 - Antibody response
AU - Zeiss, C. Raymond
AU - Levitz, Doris
AU - Leach, Chester L.
AU - Hatoum, Nabil S.
AU - Ratajczak, Helen V.
AU - Chandler, Michael J.
AU - Roger, Jean Claude
AU - Garvin, Paul J.
N1 - Funding Information:
From the Veterans Administration Lakeside Medical Center, the Department of Medicine, Section of Allergy-lmmunology, Northwestern University Medical School, *liT Research Insti-tute, Life Sciences Research, and the **Amoco Corporation. Environmental Affairs and Safety Department, Chicago, I11. Supported by the Veterans Administration (Medical Research Ser-vice) and the Amoco Corporation, Environmental Affairs and Safety Department. Received for publication March 25, 1986. Accepted for publication June 25. 1986. Reprint requests: C. R. Zeiss. M.D., ACOS Research and Devel-opment, VA Lakeside Medical Center, 400 E. Ontario, Chicago, IL 60611.
PY - 1987/1
Y1 - 1987/1
N2 - We studied lung injury induced in Sprague-Dawley rats by trimellitic anhydride (TMA) inhalation. Groups of 40 male and 20 female rats were exposed to TMA by inhalation at target concentrations of 0, 10, 30, 100, and 300 μg/m3, 6 hours per day, 5 days per week, for 2 weeks. Rats in each exposure group were sacrificed after 10 exposures or rested for 12 days and either sacrificed or received a 6-hour TMA challenge at their initial exposure levels and sacrificed at 24 hours. At each sacrifice, serum antibody to radiolabeled trimellityl rat serum albumin (RSA-TM) was measured by an ammonium sulfate technique, and lung pathology was determined. After 10 days of exposure, external hemorrhagic lung foci were directly related to the exposure concentration of TMA. Serum antibody binding of RSA-TM correlated with exposure concentration, hemorrhagic lung foci, and lung weight. There was healing of lung lesions 12 days after exposure with a return of lung lesions only 18 hours after the 6-hour inhalation challenge. A correlation between serum antibody to RSA-TM, hemorrhagic foci, and lung weight existed after challenge. This model clarifies two clinical entities observed in exposed workers, the late respiratory systemic and the pulmonary disease-anemia syndromes.
AB - We studied lung injury induced in Sprague-Dawley rats by trimellitic anhydride (TMA) inhalation. Groups of 40 male and 20 female rats were exposed to TMA by inhalation at target concentrations of 0, 10, 30, 100, and 300 μg/m3, 6 hours per day, 5 days per week, for 2 weeks. Rats in each exposure group were sacrificed after 10 exposures or rested for 12 days and either sacrificed or received a 6-hour TMA challenge at their initial exposure levels and sacrificed at 24 hours. At each sacrifice, serum antibody to radiolabeled trimellityl rat serum albumin (RSA-TM) was measured by an ammonium sulfate technique, and lung pathology was determined. After 10 days of exposure, external hemorrhagic lung foci were directly related to the exposure concentration of TMA. Serum antibody binding of RSA-TM correlated with exposure concentration, hemorrhagic lung foci, and lung weight. There was healing of lung lesions 12 days after exposure with a return of lung lesions only 18 hours after the 6-hour inhalation challenge. A correlation between serum antibody to RSA-TM, hemorrhagic foci, and lung weight existed after challenge. This model clarifies two clinical entities observed in exposed workers, the late respiratory systemic and the pulmonary disease-anemia syndromes.
UR - http://www.scopus.com/inward/record.url?scp=0023158933&partnerID=8YFLogxK
U2 - 10.1016/S0091-6749(87)80017-9
DO - 10.1016/S0091-6749(87)80017-9
M3 - Article
C2 - 3805548
AN - SCOPUS:0023158933
SN - 0091-6749
VL - 79
SP - 59
EP - 63
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 1
ER -