A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry

Alan Wells, Harald Steen, Sem Saeland, Tore Godal, George Klein

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.

Original languageEnglish
Pages (from-to)127-136
Number of pages10
JournalJournal of Virological Methods
Volume3
Issue number3
DOIs
StatePublished - Oct 1981
Externally publishedYes

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