A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry

Alan Wells; Harald Steen; Sem Saeland; Tore Godal; George Klein

doi:10.1016/0166-0934(81)90047-1

A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry

Alan Wells
, Harald Steen
, Sem Saeland
, Tore Godal
, George Klein

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.

Original language	English
Pages (from-to)	127-136
Number of pages	10
Journal	Journal of Virological Methods
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/0166-0934(81)90047-1
State	Published - Oct 1981
Externally published	Yes

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10.1016/0166-0934(81)90047-1

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title = "A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry",

abstract = "A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.",

author = "Alan Wells and Harald Steen and Sem Saeland and Tore Godal and George Klein",

note = "Funding Information: We wish to expresso ur sincereg ratitudet o Ms. Mona Hedenskoga nd Ms. Kari Hildrum for their excellent technicaasl sistancea nd Mrs. Anita Lofgren for typing the manuscript. A.W. is a recipient of an ITT International Fellowship and a fellowship from Concern Foundation. H.S. is supportedb y the Norwegian Cancer Society. Funding Information: This investigation was supported by Grant Number 2 RO 1C A 14054-07A1,a warded by the National Cancer Institute, DHEW, the Swedish Cancer Society and King Gustav V Jubilee Fund and the Norwegian Cancer Society.",

year = "1981",

month = oct,

doi = "10.1016/0166-0934(81)90047-1",

language = "English",

volume = "3",

pages = "127--136",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry

AU - Wells, Alan

AU - Steen, Harald

AU - Saeland, Sem

AU - Godal, Tore

AU - Klein, George

N1 - Funding Information: We wish to expresso ur sincereg ratitudet o Ms. Mona Hedenskoga nd Ms. Kari Hildrum for their excellent technicaasl sistancea nd Mrs. Anita Lofgren for typing the manuscript. A.W. is a recipient of an ITT International Fellowship and a fellowship from Concern Foundation. H.S. is supportedb y the Norwegian Cancer Society. Funding Information: This investigation was supported by Grant Number 2 RO 1C A 14054-07A1,a warded by the National Cancer Institute, DHEW, the Swedish Cancer Society and King Gustav V Jubilee Fund and the Norwegian Cancer Society.

PY - 1981/10

Y1 - 1981/10

N2 - A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.

AB - A quantitative microassay for detecting and analyzing the Epstein-Barr virus receptor (EBVR) utilizing fluorescein-conjugated virions is presented. The test is virus substrain-specific. Both the B95-8 and P3HR-1 strains were labelled and adsorbed to a variety of targets. Relative binding of virus was assessed by flow cytometry, the results being directly comparable with those obtained by earlier methods. Cell size and cellular DNA content were measured simultaneously with virus binding, thus enabling us to calculate EBVR density and to correlate receptor synthesis and cell cycle stage.

UR - https://www.scopus.com/pages/publications/0019855282

U2 - 10.1016/0166-0934(81)90047-1

DO - 10.1016/0166-0934(81)90047-1

M3 - Article

C2 - 6271799

AN - SCOPUS:0019855282

SN - 0166-0934

VL - 3

SP - 127

EP - 136

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 3

ER -

A microassay for quantitatively detecting the epstein-barr virus receptor on single cells utilizing flow cytometry

Abstract

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