TY - JOUR
T1 - A method to measure cellular adhesion utilizing a polymer micro-cantilever
AU - Gaitas, Angelo
AU - Malhotra, Ricky
AU - Pienta, Kenneth
N1 - Funding Information:
The authors thank Dr. T. Li and Dr. W. Zhu for help with the set-up, the micro-cantilever fabrication, and preparation, Dr. H. Roca and Dr. S. Sud in Prof. Pienta's laboratory for help with cell culture and materials, Dr. S. Akiyama for his valuable feedback. The micro-cantilevers were fabricated at the Lurie Nanofabrication Facility of the University of Michigan, Ann Arbor. The work of was partially supported by the National Institutes of Health (Grant No. GM084520).
PY - 2013/9/16
Y1 - 2013/9/16
N2 - In the present study we engineered a micro-machined polyimide cantilever with an embedded sensing element to investigate cellular adhesion, in terms of its relative ability to stick to a cross-linker, 3,3′- dithiobis[sulfosuccinimidylpropionate], coated on the cantilever surface. To achieve this objective, we investigated adhesive properties of three human prostate cancer cell lines, namely, a bone metastasis derived human prostate cancer cell line (PC3), a brain metastasis derived human prostate cancer cell line (DU145), and a subclone of PC3 (PC3-EMT14). We found that PC3-EMT14, which displays a mesenchymal phenotype, has the least adhesion compared to PC3 and DU145, which exhibit an epithelial phenotype.
AB - In the present study we engineered a micro-machined polyimide cantilever with an embedded sensing element to investigate cellular adhesion, in terms of its relative ability to stick to a cross-linker, 3,3′- dithiobis[sulfosuccinimidylpropionate], coated on the cantilever surface. To achieve this objective, we investigated adhesive properties of three human prostate cancer cell lines, namely, a bone metastasis derived human prostate cancer cell line (PC3), a brain metastasis derived human prostate cancer cell line (DU145), and a subclone of PC3 (PC3-EMT14). We found that PC3-EMT14, which displays a mesenchymal phenotype, has the least adhesion compared to PC3 and DU145, which exhibit an epithelial phenotype.
UR - https://www.scopus.com/pages/publications/84884861135
U2 - 10.1063/1.4821946
DO - 10.1063/1.4821946
M3 - Article
AN - SCOPUS:84884861135
SN - 0003-6951
VL - 103
JO - Applied Physics Letters
JF - Applied Physics Letters
IS - 12
M1 - 123702
ER -