A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Mark J. Bailey, Felix Broecker, Paul E. Leon, Gene S. Tan

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Antibodies play a crucial role in coupling the innate and adaptive immune responses against viral pathogens through their antigen binding domains and Fc-regions. Here, we describe how to measure the activation of Fc effector functions by monoclonal antibodies targeting the influenza virus hemagglutinin with the use of a genetically engineered Jurkat cell line expressing an activating type 1 Fc-FcγR. Using this method, the contribution of specific Fc-FcγR interactions conferred by immunoglobulins can be determined using an in vitro assay.

Original languageEnglish
Article numbere56256
JournalJournal of Visualized Experiments
Volume2018
Issue number132
DOIs
StatePublished - 2018

Keywords

  • Immunology
  • Issue 132

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