TY - JOUR
T1 - A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family
AU - Stamenkovic, Ivan
AU - Amiot, Martine
AU - Pesando, John M.
AU - Seed, Brian
N1 - Funding Information:
We thank Ralph Budd for a generous gift of the IM7 monoclonal antibody, Bart Haynes, Lloyd Stoolman, and Eugene Butcher for advice and helpful discussions, and Larry Lasky for sharing Mel-14 sequence data. Ivan Stamenkovic is a Special Fellow of the Leukemia Society of America. This work was supported by a grant from Hoechst AG.
PY - 1989/3/24
Y1 - 1989/3/24
N2 - Monoclonal antibodies in the Hermes family recognize a lymphocyte structure that participates in lymphocyte adhesion to endothelium and has been suggested to be the human homolog of the murine Mel-14 lymph node homing receptor. Recently, antibodies against the Hermes antigen, the polymorphic glycoprotein Pgp-1 antigen, and the broadly expressed CDw44 antigen have been shown to recognize the same structure. In this work, cDNA clones encoding the CDw44 antigen were isolated and expressed in COS cells. Two forms were identified: a lymphoid form expressed in hematopoietic cells, and an epithelial form weakly expressed in normal epithelium but highly expressed in carcinomas. The extracellular domain of CDw44 bears homology to cartilage link proteins and a related segment of proteoglycan core protein. However, comparison with the recently identified sequence of the Mel-14 antigen shows that CDw44 and Mel-14 are unrelated.
AB - Monoclonal antibodies in the Hermes family recognize a lymphocyte structure that participates in lymphocyte adhesion to endothelium and has been suggested to be the human homolog of the murine Mel-14 lymph node homing receptor. Recently, antibodies against the Hermes antigen, the polymorphic glycoprotein Pgp-1 antigen, and the broadly expressed CDw44 antigen have been shown to recognize the same structure. In this work, cDNA clones encoding the CDw44 antigen were isolated and expressed in COS cells. Two forms were identified: a lymphoid form expressed in hematopoietic cells, and an epithelial form weakly expressed in normal epithelium but highly expressed in carcinomas. The extracellular domain of CDw44 bears homology to cartilage link proteins and a related segment of proteoglycan core protein. However, comparison with the recently identified sequence of the Mel-14 antigen shows that CDw44 and Mel-14 are unrelated.
UR - http://www.scopus.com/inward/record.url?scp=0024560984&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(89)90638-7
DO - 10.1016/0092-8674(89)90638-7
M3 - Article
C2 - 2466575
AN - SCOPUS:0024560984
SN - 0092-8674
VL - 56
SP - 1057
EP - 1062
JO - Cell
JF - Cell
IS - 6
ER -