A Long Noncoding RNA Regulates Sister Chromatid Cohesion

Francesco P. Marchese, Elena Grossi, Oskar Marín-Béjar, Sanjay Kumar Bharti, Ivan Raimondi, Jovanna González, Dannys Jorge Martínez-Herrera, Alejandro Athie, Alicia Amadoz, Robert M. Brosh, Maite Huarte

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication and sister chromatid cohesion. These findings unveil a direct role for an lncRNA in the establishment of sister chromatid cohesion by modulating DDX11 enzymatic activity.

Original languageEnglish
Pages (from-to)397-407
Number of pages11
JournalMolecular Cell
Volume63
Issue number3
DOIs
StatePublished - 4 Aug 2016
Externally publishedYes

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