A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

Charles E. Burnham; Bruce Buerk; Charles Schmidt; John C. Bucuvalas

doi:10.1016/0005-2736(96)00118-6

A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

Charles E. Burnham
, Bruce Buerk
, Charles Schmidt
, John C. Bucuvalas

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We report the cloning of a 2.2 kb cDNA encoding a Na⁺- and Cl^--dependent betaine/GABA (γ-aminobutyric acid) transporter from rat liver poly(A⁺) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe, Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

Original language	English
Pages (from-to)	4-8
Number of pages	5
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1284
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(96)00118-6
State	Published - 2 Oct 1996
Externally published	Yes

Keywords

Betaine
Membrane transporter
Osmolyte

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10.1016/0005-2736(96)00118-6

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abstract = "We report the cloning of a 2.2 kb cDNA encoding a Na+- and Cl--dependent betaine/GABA (γ-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe, Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.",

keywords = "Betaine, Membrane transporter, Osmolyte",

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AU - Buerk, Bruce

AU - Schmidt, Charles

AU - Bucuvalas, John C.

PY - 1996/10/2

Y1 - 1996/10/2

N2 - We report the cloning of a 2.2 kb cDNA encoding a Na+- and Cl--dependent betaine/GABA (γ-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe, Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

AB - We report the cloning of a 2.2 kb cDNA encoding a Na+- and Cl--dependent betaine/GABA (γ-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe, Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

KW - Betaine

KW - Membrane transporter

KW - Osmolyte

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JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 1

ER -

A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

Abstract

Keywords

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