A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

Charles E. Burnham, Bruce Buerk, Charles Schmidt, John C. Bucuvalas

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We report the cloning of a 2.2 kb cDNA encoding a Na+- and Cl--dependent betaine/GABA (γ-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe, Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

Original languageEnglish
Pages (from-to)4-8
Number of pages5
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1284
Issue number1
DOIs
StatePublished - 2 Oct 1996
Externally publishedYes

Keywords

  • Betaine
  • Membrane transporter
  • Osmolyte

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