A large-scale retrospective study enabled deep-learning based pathological assessment of frozen procurement kidney biopsies to predict graft loss and guide organ utilization

Zhengzi Yi, Caixia Xi, Madhav C. Menon, Paolo Cravedi, Fasika Tedla, Alan Soto, Zeguo Sun, Keyu Liu, Jason Zhang, Chengguo Wei, Man Chen, Wenlin Wang, Brandon Veremis, Monica Garcia-barros, Abhishek Kumar, Danielle Haakinson, Rachel Brody, Evren U. Azeloglu, Lorenzo Gallon, Philip O'ConnellMaarten Naesens, Ron Shapiro, Robert B. Colvin, Stephen Ward, Fadi Salem, Weijia Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Lesion scores on procurement donor biopsies are commonly used to guide organ utilization for deceased-donor kidneys. However, frozen sections present challenges for histological scoring, leading to inter- and intra-observer variability and inappropriate discard. Therefore, we constructed deep-learning based models to recognize kidney tissue compartments in hematoxylin & eosin-stained sections from procurement needle biopsies performed nationwide in years 2011-2020. To do this, we extracted whole-slide abnormality features from 2431 kidneys and correlated with pathologists’ scores and transplant outcomes. A Kidney Donor Quality Score (KDQS) was derived and used in combination with recipient demographic and peri-transplant characteristics to predict graft loss or assist organ utilization. The performance on wedge biopsies was additionally evaluated. Our model identified 96% and 91% of normal/sclerotic glomeruli respectively; 94% of arteries/arterial intimal fibrosis; 90% of tubules. Whole-slide features of Sclerotic Glomeruli (GS)%, Arterial Intimal Fibrosis (AIF)%, and Interstitial Space Abnormality (ISA)% demonstrated strong correlations with corresponding pathologists’ scores of all 2431 kidneys, but had superior associations with post-transplant estimated glomerular filtration rates in 2033 and graft loss in 1560 kidneys. The combination of KDQS and other factors predicted one- and four-year graft loss in a discovery set of 520 kidneys and a validation set of 1040 kidneys. By using the composite KDQS of 398 discarded kidneys due to “biopsy findings”, we suggest that if transplanted, 110 discarded kidneys could have had similar survival to that of other transplanted kidneys. Thus, our composite KDQS and survival prediction models may facilitate risk stratification and organ utilization while potentially reducing unnecessary organ discard.

Original languageEnglish
Pages (from-to)281-292
Number of pages12
JournalKidney International
Volume105
Issue number2
DOIs
StatePublished - Feb 2024

Keywords

  • artificial intelligence
  • kidney biopsy
  • renal pathology
  • transplantation

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