A Human‐Human Monoclonal Anti‐D by Direct Fusion with a Lymphoblastoid Line

A. D. Lowe; S. M. Green; D. Voak; T. Gibson; E. S. Lennox

doi:10.1111/j.1423-0410.1986.tb01955.x

A Human‐Human Monoclonal Anti‐D by Direct Fusion with a Lymphoblastoid Line

A. D. Lowe, S. M. Green, D. Voak, T. Gibson, E. S. Lennox

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Abstract. The human lymphoblastoid cell line W1‐L2–729‐HF₂ has been fused with B cells from a plasmaphoresed anti‐D donor immunized with D⁺ cells. A stable monoclonal antibody‐producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. D^u red cells are detected in enzyme enhanced tests.

Original language	English
Pages (from-to)	212-216
Number of pages	5
Journal	Vox Sanguinis
Volume	51
Issue number	3
DOIs	https://doi.org/10.1111/j.1423-0410.1986.tb01955.x
State	Published - Nov 1986
Externally published	Yes

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abstract = "Abstract. The human lymphoblastoid cell line W1‐L2–729‐HF2 has been fused with B cells from a plasmaphoresed anti‐D donor immunized with D+ cells. A stable monoclonal antibody‐producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.",

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N2 - Abstract. The human lymphoblastoid cell line W1‐L2–729‐HF2 has been fused with B cells from a plasmaphoresed anti‐D donor immunized with D+ cells. A stable monoclonal antibody‐producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.

AB - Abstract. The human lymphoblastoid cell line W1‐L2–729‐HF2 has been fused with B cells from a plasmaphoresed anti‐D donor immunized with D+ cells. A stable monoclonal antibody‐producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.

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A Human‐Human Monoclonal Anti‐D by Direct Fusion with a Lymphoblastoid Line

Abstract

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