A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

Vera Alexeeva, Sunita L. D'Souza, Christoph Schaniel

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1+ cell populations.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalStem Cell Research
Volume17
Issue number1
DOIs
StatePublished - 1 Jul 2016

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