A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing

Vera Alexeeva; Iraz T. Aydin; Christoph Schaniel; Alec W. Stranahan; Sunita L. D'Souza; James J. Bieker

doi:10.1016/j.scr.2020.101837

A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing

Vera Alexeeva, Iraz T. Aydin, Christoph Schaniel, Alec W. Stranahan, Sunita L. D'Souza, James J. Bieker

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.

Original language	English
Article number	101837
Journal	Stem Cell Research
Volume	45
DOIs	https://doi.org/10.1016/j.scr.2020.101837
State	Published - May 2020

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title = "A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing",

abstract = "Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and {\ss}-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and {\ss}-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.",

author = "Vera Alexeeva and Aydin, {Iraz T.} and Christoph Schaniel and Stranahan, {Alec W.} and D'Souza, {Sunita L.} and Bieker, {James J.}",

note = "Funding Information: This work was supported by NIH grant RC1 DK086200 and NYSTEM grant C026435 to JJB. Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = may,

doi = "10.1016/j.scr.2020.101837",

language = "English",

volume = "45",

journal = "Stem Cell Research",

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T1 - A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing

AU - Alexeeva, Vera

AU - Aydin, Iraz T.

AU - Schaniel, Christoph

AU - Stranahan, Alec W.

AU - D'Souza, Sunita L.

AU - Bieker, James J.

PY - 2020/5

Y1 - 2020/5

N2 - Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.

AB - Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. However, hESC-derived red cells exhibit an embryonic/fetal, but not adult, mode of hemoglobin expression. The resource described here is a hESC line engineered to express a reporter from its adult globin promoter, providing a screening platform for small molecules that lead to efficient induction of adult globin.

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A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing

Abstract

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