A highly specific and sensitive enzyme-immunometric assay for calcitonin gene-related peptide based on enzyme amplification

Rashmi Seth; Mone Zaidi; James Q. Fuller; Colin H. Self

doi:10.1016/0022-1759(88)90053-1

A highly specific and sensitive enzyme-immunometric assay for calcitonin gene-related peptide based on enzyme amplification

Rashmi Seth, Mone Zaidi, James Q. Fuller, Colin H. Self

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Calcitonin gene-related peptide (CGRP) is an important member of the peptide family encoded by the calcitonin gene. It has been found to be a potent vasodilator in man and a major circulating gene product (Girgis et al., 1985). The present study reports the development of a sensitive and rapid two-site immunoassay for CGRP based on enzyme amplification (Self, 1985). The assay has been easy to construct, taking advantage of available antisera raised for other purposes. Nevertheless it has been found to be clearly superior to our previous radioimmunoassay in terms of sensitivity, specificity, speed and convenience.

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Journal of Immunological Methods
Volume	111
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(88)90053-1
State	Published - 28 Jun 1988
Externally published	Yes

Keywords

Amplified immunometric assay
Calcitonin gene-related peptide
Enzyme amplification
Peptide immunoassay

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10.1016/0022-1759(88)90053-1

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title = "A highly specific and sensitive enzyme-immunometric assay for calcitonin gene-related peptide based on enzyme amplification",

abstract = "Calcitonin gene-related peptide (CGRP) is an important member of the peptide family encoded by the calcitonin gene. It has been found to be a potent vasodilator in man and a major circulating gene product (Girgis et al., 1985). The present study reports the development of a sensitive and rapid two-site immunoassay for CGRP based on enzyme amplification (Self, 1985). The assay has been easy to construct, taking advantage of available antisera raised for other purposes. Nevertheless it has been found to be clearly superior to our previous radioimmunoassay in terms of sensitivity, specificity, speed and convenience.",

keywords = "Amplified immunometric assay, Calcitonin gene-related peptide, Enzyme amplification, Peptide immunoassay",

author = "Rashmi Seth and Mone Zaidi and Fuller, {James Q.} and Self, {Colin H.}",

note = "Funding Information: We are thankful to the MRC. MZ acknowledges the support of the Council of Vice-Chancellors and Principals of the Universities of the U.K. and the Lady Tata Memorial Trust, London.",

year = "1988",

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doi = "10.1016/0022-1759(88)90053-1",

language = "English",

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TY - JOUR

T1 - A highly specific and sensitive enzyme-immunometric assay for calcitonin gene-related peptide based on enzyme amplification

AU - Seth, Rashmi

AU - Zaidi, Mone

AU - Fuller, James Q.

AU - Self, Colin H.

N1 - Funding Information: We are thankful to the MRC. MZ acknowledges the support of the Council of Vice-Chancellors and Principals of the Universities of the U.K. and the Lady Tata Memorial Trust, London.

PY - 1988/6/28

Y1 - 1988/6/28

N2 - Calcitonin gene-related peptide (CGRP) is an important member of the peptide family encoded by the calcitonin gene. It has been found to be a potent vasodilator in man and a major circulating gene product (Girgis et al., 1985). The present study reports the development of a sensitive and rapid two-site immunoassay for CGRP based on enzyme amplification (Self, 1985). The assay has been easy to construct, taking advantage of available antisera raised for other purposes. Nevertheless it has been found to be clearly superior to our previous radioimmunoassay in terms of sensitivity, specificity, speed and convenience.

AB - Calcitonin gene-related peptide (CGRP) is an important member of the peptide family encoded by the calcitonin gene. It has been found to be a potent vasodilator in man and a major circulating gene product (Girgis et al., 1985). The present study reports the development of a sensitive and rapid two-site immunoassay for CGRP based on enzyme amplification (Self, 1985). The assay has been easy to construct, taking advantage of available antisera raised for other purposes. Nevertheless it has been found to be clearly superior to our previous radioimmunoassay in terms of sensitivity, specificity, speed and convenience.

KW - Amplified immunometric assay

KW - Calcitonin gene-related peptide

KW - Enzyme amplification

KW - Peptide immunoassay

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U2 - 10.1016/0022-1759(88)90053-1

DO - 10.1016/0022-1759(88)90053-1

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

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ER -

A highly specific and sensitive enzyme-immunometric assay for calcitonin gene-related peptide based on enzyme amplification

Abstract

Keywords

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