A high-affinity monoclonal antibody (Gif-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2΄-5΄ (A) synthetase induction

Carl Feit; Ganes Sen; Arie H. Bartal; Yashar Hirshaut; Sylvia L. Anderson; Susan K. Millet; Berish Y. Rubin

doi:10.1159/000163357

A high-affinity monoclonal antibody (Gif-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2΄-5΄ (A) synthetase induction

Carl Feit
, Ganes Sen
, Arie H. Bartal
, Yashar Hirshaut
, Sylvia L. Anderson
, Susan K. Millet
, Berish Y. Rubin

Research output: Contribution to journal › Article › peer-review

Abstract

An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-γ) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-γ, the ability of HuIFN-γ to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-γ to induce the 2΄-5΄ oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE.

Original language	English
Pages (from-to)	212-219
Number of pages	8
Journal	Pathobiology
Volume	54
Issue number	4
DOIs	https://doi.org/10.1159/000163357
State	Published - 1986
Externally published	Yes

Keywords

2΄-5΄ (A) Synthetase
Interferons
Monoclonal antibody
Retroviruses

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10.1159/000163357

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title = "A high-affinity monoclonal antibody (Gif-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2΄-5΄ (A) synthetase induction",

abstract = "An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-γ) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-γ, the ability of HuIFN-γ to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-γ to induce the 2΄-5΄ oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE.",

keywords = "2΄-5΄ (A) Synthetase, Interferons, Monoclonal antibody, Retroviruses",

author = "Carl Feit and Ganes Sen and Bartal, \{Arie H.\} and Yashar Hirshaut and Anderson, \{Sylvia L.\} and Millet, \{Susan K.\} and Rubin, \{Berish Y.\}",

year = "1986",

doi = "10.1159/000163357",

language = "English",

volume = "54",

pages = "212--219",

journal = "Pathobiology",

issn = "1015-2008",

publisher = "S. Karger AG",

number = "4",

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TY - JOUR

T1 - A high-affinity monoclonal antibody (Gif-1) to human gamma-interferon

T2 - Neutralization of interferon mediated inhibition of retrovirus production and 2΄-5΄ (A) synthetase induction

AU - Feit, Carl

AU - Sen, Ganes

AU - Bartal, Arie H.

AU - Hirshaut, Yashar

AU - Anderson, Sylvia L.

AU - Millet, Susan K.

AU - Rubin, Berish Y.

PY - 1986

Y1 - 1986

N2 - An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-γ) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-γ, the ability of HuIFN-γ to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-γ to induce the 2΄-5΄ oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE.

AB - An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-γ) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-γ, the ability of HuIFN-γ to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-γ to induce the 2΄-5΄ oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE.

KW - 2΄-5΄ (A) Synthetase

KW - Interferons

KW - Monoclonal antibody

KW - Retroviruses

UR - https://www.scopus.com/pages/publications/84940626317

U2 - 10.1159/000163357

DO - 10.1159/000163357

M3 - Article

C2 - 3023159

AN - SCOPUS:84940626317

SN - 1015-2008

VL - 54

SP - 212

EP - 219

JO - Pathobiology

JF - Pathobiology

IS - 4

ER -

A high-affinity monoclonal antibody (Gif-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2΄-5΄ (A) synthetase induction

Abstract

Keywords

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