A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

H. Granok; B. A. Leibovitch; S. C.R. Elgin

doi:10.1017/S0016672301005122

A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

H. Granok
, B. A. Leibovitch
, S. C.R. Elgin

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.

Original language	English
Pages (from-to)	13-21
Number of pages	9
Journal	Genetical Research
Volume	78
Issue number	1
DOIs	https://doi.org/10.1017/S0016672301005122
State	Published - 2001
Externally published	Yes

Access to Document

10.1017/S0016672301005122

Cite this

@article{674137d531d34875b78965f92206b837,

title = "A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene",

abstract = "GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.",

author = "H. Granok and Leibovitch, \{B. A.\} and Elgin, \{S. C.R.\}",

year = "2001",

doi = "10.1017/S0016672301005122",

language = "English",

volume = "78",

pages = "13--21",

journal = "Genetical Research",

issn = "0016-6723",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

AU - Granok, H.

AU - Leibovitch, B. A.

AU - Elgin, S. C.R.

PY - 2001

Y1 - 2001

N2 - GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.

AB - GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.

UR - https://www.scopus.com/pages/publications/0034888980

U2 - 10.1017/S0016672301005122

DO - 10.1017/S0016672301005122

M3 - Article

C2 - 11556133

AN - SCOPUS:0034888980

SN - 0016-6723

VL - 78

SP - 13

EP - 21

JO - Genetical Research

JF - Genetical Research

IS - 1

ER -

A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

Abstract

Access to Document

Fingerprint

Cite this