A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background

Richard C. Davis; Eric E. Schadt; Desmond J. Smith; Elena W.Y. Hsieh; Alessandra C.L. Cervino; Atila Van Nas; Melenie Rosales; Sudheer Doss; Haijin Meng; Hooman Allayee; Aldons J. Lusis

doi:10.1016/j.ygeno.2005.05.010

A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background

Richard C. Davis, Eric E. Schadt, Desmond J. Smith, Elena W.Y. Hsieh, Alessandra C.L. Cervino, Atila Van Nas, Melenie Rosales, Sudheer Doss, Haijin Meng, Hooman Allayee, Aldons J. Lusis

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30 Scopus citations

Abstract

In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by "Mendelizing" the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains.

Original language	English
Pages (from-to)	259-270
Number of pages	12
Journal	Genomics
Volume	86
Issue number	3
DOIs	https://doi.org/10.1016/j.ygeno.2005.05.010
State	Published - Sep 2005
Externally published	Yes

Keywords

Chromosome mapping
Congenic mice
Gene mapping
Genetic polymorphism
Genetics
Genome mapping
Laboratory mouse
Single nucleotide polymorphism

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10.1016/j.ygeno.2005.05.010

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title = "A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background",

abstract = "In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by {"}Mendelizing{"} the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains.",

keywords = "Chromosome mapping, Congenic mice, Gene mapping, Genetic polymorphism, Genetics, Genome mapping, Laboratory mouse, Single nucleotide polymorphism",

author = "Davis, {Richard C.} and Schadt, {Eric E.} and Smith, {Desmond J.} and Hsieh, {Elena W.Y.} and Cervino, {Alessandra C.L.} and {Van Nas}, Atila and Melenie Rosales and Sudheer Doss and Haijin Meng and Hooman Allayee and Lusis, {Aldons J.}",

note = "Funding Information: This work was carried out with funds from NIH Grants HL30568, HL28481, HL60030 and HL70526 and generous support from Rosetta Inpharmatics/Merck Inc. ",

year = "2005",

month = sep,

doi = "10.1016/j.ygeno.2005.05.010",

language = "English",

volume = "86",

pages = "259--270",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "3",

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T1 - A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background

AU - Davis, Richard C.

AU - Schadt, Eric E.

AU - Smith, Desmond J.

AU - Hsieh, Elena W.Y.

AU - Cervino, Alessandra C.L.

AU - Van Nas, Atila

AU - Rosales, Melenie

AU - Doss, Sudheer

AU - Meng, Haijin

AU - Allayee, Hooman

AU - Lusis, Aldons J.

N1 - Funding Information: This work was carried out with funds from NIH Grants HL30568, HL28481, HL60030 and HL70526 and generous support from Rosetta Inpharmatics/Merck Inc.

PY - 2005/9

Y1 - 2005/9

N2 - In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by "Mendelizing" the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains.

AB - In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by "Mendelizing" the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains.

KW - Chromosome mapping

KW - Congenic mice

KW - Gene mapping

KW - Genetic polymorphism

KW - Genetics

KW - Genome mapping

KW - Laboratory mouse

KW - Single nucleotide polymorphism

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DO - 10.1016/j.ygeno.2005.05.010

M3 - Article

C2 - 16039824

AN - SCOPUS:23244452256

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VL - 86

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EP - 270

JO - Genomics

JF - Genomics

IS - 3

ER -

A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background

Abstract

Keywords

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