A feasible method for As speciation in several types of seafood by LC-ICP-MS/MS

  • Lucas Schmidt
  • , Julio Alberto Landero
  • , Diogo La Rosa Novo
  • , Fabio Andrei Duarte
  • , Marcia Foster Mesko
  • , Joseph A. Caruso
  • , Erico Marlon Moraes Flores

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

A method for arsenic speciation in shark, shrimp, squid, oyster and scallop using liquid chromatography coupled to inductively coupled plasma triple quadrupole mass spectrometry (LC-ICP-MS/MS) was proposed. Suitable sensitivity and selectivity by LC-ICP-MS/MS were obtained using 10 mmol L−1 (NH4)2HPO4 diluted in 1% methanol (pH 8.65) as mobile phase. Recoveries from 90 to 104% for arsenobetaine (AsB), arsenite [As(III)], dimethylarsinic acid (DMA), monomethylarsonic acid (MMA) and arsenate [As(V)] were obtained for all samples. A certificated reference material was also analyzed and the sum of As species was in agreement with the total As concentration. Limits of quantification (LOQ) for AsB, As(III), DMA, MMA, and As(V) were 6, 30, 6, 12 and 26 ng g−1, respectively. Higher concentration of AsB was found in all seafood, while As(III) and DMA were found only in oyster. Arsenate was found in squid and scallops, and MMA was below the LOQ in all samples.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalFood Chemistry
Volume255
DOIs
StatePublished - 30 Jul 2018
Externally publishedYes

Keywords

  • Arsenic speciation
  • Extraction
  • Food safety
  • Inductively coupled plasma triple quadrupole mass spectrometry
  • Liquid chromatography
  • Sample preparation

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