TY - JOUR
T1 - A double determinant immunoassay for HLA class I typing using serum as an antigen source
AU - Russo, Carlo
AU - Fotino, Marilena
AU - Carbonara, Angelo
AU - Ferrone, Soldano
N1 - Funding Information:
Kidney C~t~ Comdl Unitersity Medical Cgkge; D~p~t of Mdicd G~tics Uni'~rsity ~f Txrin Medical Sd~l, Italy; and Dc~t of Mio~ido~ and Immzne~gy, New York Mcdic~dC ¢~e, Vat-bM~, New York. "This work waJ sNpBortedi n part by National Institute of Health Grants CA 38407, CA 38469, and BRSG 2 807 RR05396. C.R. is a ncipknt o/ a William S. Paley Fallen/shipi n AcademicM ulicine. Addms reprint requestst o Dr. CarloR usso, D,partmento fMulicine, Ceradl Uni~i~ MWlicalC dlg¢, 1300 Yof~ Avenue, New York, NY 10021. Reuiv¢d A~tgust 20, 1986; aceeptulJanuary 28, 1987.
PY - 1987/5
Y1 - 1987/5
N2 - We have applied a double determinant immunoassay (DDIA) to HLA-A2,A28, and B13 typing, using serum as an antigen source. The results obtained show a correlation of 96% (B13) and 89.1% (A2,A28) with the results obtained by conventional HLA typing. Furthermore, the results obtained were highly reproducible, since testing of 18 sera on two occasions gave concordant results with all samples tested. The variation in the content of HLA-A2 antigens in sera taken at different times from a given donor was less than 5%. A sevenfold variation was found in the serum level of HLA-A2,A28 antigens; the highest level was found in the sera from HLA-A2,A28 donors and in decreasing order in HLA-A2 homozygous, HLA-A28 homozygous, HLA-A2 heterozygous, and HLA-A28 heterozygous donors. The results of this study indicate that the DDIA is a sensitive, simple, and reproducible procedure for HLA class I typing. The DDIA offers the following advantages in comparison with the conventional lymphocytotoxic assay: (1) it provides information not only about the expression of a given alloantigen, but also about its level; (2) it does not require viable cells, thus facilitating retrospective studies and typing of leucopenic patients; (3) it eliminates variability of results caused by abnormal susceptibility of target cells to complement-dependent lysis.
AB - We have applied a double determinant immunoassay (DDIA) to HLA-A2,A28, and B13 typing, using serum as an antigen source. The results obtained show a correlation of 96% (B13) and 89.1% (A2,A28) with the results obtained by conventional HLA typing. Furthermore, the results obtained were highly reproducible, since testing of 18 sera on two occasions gave concordant results with all samples tested. The variation in the content of HLA-A2 antigens in sera taken at different times from a given donor was less than 5%. A sevenfold variation was found in the serum level of HLA-A2,A28 antigens; the highest level was found in the sera from HLA-A2,A28 donors and in decreasing order in HLA-A2 homozygous, HLA-A28 homozygous, HLA-A2 heterozygous, and HLA-A28 heterozygous donors. The results of this study indicate that the DDIA is a sensitive, simple, and reproducible procedure for HLA class I typing. The DDIA offers the following advantages in comparison with the conventional lymphocytotoxic assay: (1) it provides information not only about the expression of a given alloantigen, but also about its level; (2) it does not require viable cells, thus facilitating retrospective studies and typing of leucopenic patients; (3) it eliminates variability of results caused by abnormal susceptibility of target cells to complement-dependent lysis.
UR - http://www.scopus.com/inward/record.url?scp=0023178336&partnerID=8YFLogxK
U2 - 10.1016/0198-8859(87)90039-5
DO - 10.1016/0198-8859(87)90039-5
M3 - Article
C2 - 3495524
AN - SCOPUS:0023178336
SN - 0198-8859
VL - 19
SP - 69
EP - 77
JO - Human Immunology
JF - Human Immunology
IS - 1
ER -