TY - JOUR
T1 - A dose-response study following in utero and lactational exposure to di-(2-ethylhexyl) phthalate (DEHP)
T2 - Reproductive effects on adult female offspring rats
AU - Grande, Simone W.
AU - Andrade, Anderson J.M.
AU - Talsness, Chris E.
AU - Grote, Konstanze
AU - Golombiewski, Andrea
AU - Sterner-Kock, Anja
AU - Chahoud, Ibrahim
PY - 2007/1/5
Y1 - 2007/1/5
N2 - Di(2-ethylhexyl) phthalate (DEHP) is used in numerous consumer products, mainly imparting flexibility and durability to polyvinyl chloride (PVC) based plastics. It is a known reproductive and developmental toxicant in male rodents. However, data regarding effects of DEHP on female reproductive health are particularly sparse. We performed an extensive dose-response study following developmental exposure to DEHP and evaluated the effects on adult female reproductive function. Two wide ranges of doses, low and high, were tested. Female Wistar rats were treated daily with DEHP and peanut oil (vehicle control) by gavage from gestation day 6 to lactation day 21. The low doses were: 0.015, 0.045, 0.135, 0.405 and 1.215 mg DEHP/kg/bw/day and the high doses were: 5, 15, 45, 135 and 405 mg DEHP/kg/bw/day. At the doses tested, no effects on organ (liver, kidney, spleen, thymus, thyroid, ovary and uterus) or body weights were detected. Female offspring presented a normal pattern of estrous cyclicity with no hormonal alterations (serum estradiol and progesterone). A statistically significant increase in tertiary atretic follicles was observed at the highest dose (405 mg DEHP/kg/day). Morphometric analysis indicated that uterus and vagina luminal epithelial cell height were unaffected by treatment. An increase in the number of ovarian atretic tertiary follicles was the only effect observed in adult female offspring exposed in utero and during lactation to DEHP.
AB - Di(2-ethylhexyl) phthalate (DEHP) is used in numerous consumer products, mainly imparting flexibility and durability to polyvinyl chloride (PVC) based plastics. It is a known reproductive and developmental toxicant in male rodents. However, data regarding effects of DEHP on female reproductive health are particularly sparse. We performed an extensive dose-response study following developmental exposure to DEHP and evaluated the effects on adult female reproductive function. Two wide ranges of doses, low and high, were tested. Female Wistar rats were treated daily with DEHP and peanut oil (vehicle control) by gavage from gestation day 6 to lactation day 21. The low doses were: 0.015, 0.045, 0.135, 0.405 and 1.215 mg DEHP/kg/bw/day and the high doses were: 5, 15, 45, 135 and 405 mg DEHP/kg/bw/day. At the doses tested, no effects on organ (liver, kidney, spleen, thymus, thyroid, ovary and uterus) or body weights were detected. Female offspring presented a normal pattern of estrous cyclicity with no hormonal alterations (serum estradiol and progesterone). A statistically significant increase in tertiary atretic follicles was observed at the highest dose (405 mg DEHP/kg/day). Morphometric analysis indicated that uterus and vagina luminal epithelial cell height were unaffected by treatment. An increase in the number of ovarian atretic tertiary follicles was the only effect observed in adult female offspring exposed in utero and during lactation to DEHP.
KW - Di-(2-ethylhexyl) phthalate
KW - Dose-response
KW - Endocrine disruptors
KW - Female offspring
UR - http://www.scopus.com/inward/record.url?scp=33845426334&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2006.10.005
DO - 10.1016/j.tox.2006.10.005
M3 - Article
C2 - 17098345
AN - SCOPUS:33845426334
SN - 0300-483X
VL - 229
SP - 114
EP - 122
JO - Toxicology
JF - Toxicology
IS - 1-2
ER -