@article{ff6303f6af79430292bcb9776678f298,
title = "A direct interaction between the RAG2 C terminus and the core histones is required for efficient V(D)J recombination",
abstract = "V(D)J recombination is a tightly controlled process of somatic recombination whose regulation is mediated in part by chromatin structure. Here, we report that RAG2 binds directly to the core histone proteins. The interaction with histones is observed in developing lymphocytes and within the RAG1/RAG2 recombinase complex in a manner that is dependent on the RAG2 C terminus. Amino acids within the plant homeo domain (PHD)-like domain as well as a conserved acidic stretch of the RAG2 C terminus that is considered to be a linker region are important for this interaction. Point mutations that disrupt the RAG2-histone association inhibit the efficiency of the V(D)J recombination reaction at the endogenous immunoglobulin locus, with the most dramatic effect in the V to DJH rearrangement.",
author = "West, {Kelly L.} and Singha, {Netai C.} and {De Ioannes}, Pablo and Lynne Lacomis and Hediye Erdjument-Bromage and Paul Tempst and Patricia Cortes",
note = "Funding Information: We thank members of the Cortes lab for helpful discussion and reagents; Juan Carcamo for critically reading the manuscript; Andrea Martin, Claudia Canasta, Glaucia Furtado, and members of Dr. Sergio Lira{\textquoteright}s laboratory for help with mouse work; Adrian Ting for retroviral vectors and advice; Eva Besmer and Ziva Misulovin (The Rockefeller University, New York) for cloning vectors; and Gary A. Rathbun and Frederick W. Alt for RAG2 −/− pro-B cell lines. K.W. was supported by the National Institutes of Health (NIH) training grant AI 07605. P.T. was supported by the NCI Cancer Center Support grant P30 CA08748. Work in the P.C. laboratory is in part supported by grants AI45996 from NIH, RSG-04-191-01-LIB from the American Cancer Society, Program Project Grant PO1 AI61093, a Cancer Research Institute Investigator award, and a Leukemia and Lymphoma Society Scholar award.",
year = "2005",
month = aug,
doi = "10.1016/j.immuni.2005.07.004",
language = "English",
volume = "23",
pages = "203--212",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",
}