A Cloned cDNA Probe for Rat Immunoglobulin Epsilon Heavy Chain: Construction, Identification, and DNA Sequence

Wayne R. Kindsvogel; E. Premkumar Reddy; Joan M. Moore; Charles H. Faust

doi:10.1089/dna.1982.1.335

A Cloned cDNA Probe for Rat Immunoglobulin Epsilon Heavy Chain: Construction, Identification, and DNA Sequence

Wayne R. Kindsvogel, E. Premkumar Reddy, Joan M. Moore, Charles H. Faust

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

IgE has clinical importance because it is responsible for immediate hypersensitivity. Studies of IgE expression in rats appear to contradict current models for immunoglobulin gene expression. To study rat IgE expression at the RNA and DNA levels, we have constructed a cDNA for part of the rat epsilon (ε) heavy chain that is expressed by a rat myeloma, IR162. The rat ε-chain clone was initially identified by an efficient selection scheme. DNA sequencing of the 580-bp cDNA revealed that it encoded 176 amino acids that were 50% homologous to the human εH chain. The sequence begins near the end of the C_H2 domain and ends 31 amino acids into the C_H4 domain. Cysteines important for the structure of the human IgE were conserved in the rat εH-chain. The identity of the cloned ε cDNA was confirmed by comparison with a portion of the constant region gene for mouse εH chain. The mouse and rat nucleotide sequences were 79% homologous.

Original language	English
Pages (from-to)	335-343
Number of pages	9
Journal	DNA
Volume	1
Issue number	4
DOIs	https://doi.org/10.1089/dna.1982.1.335
State	Published - 1982
Externally published	Yes

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title = "A Cloned cDNA Probe for Rat Immunoglobulin Epsilon Heavy Chain: Construction, Identification, and DNA Sequence",

abstract = "IgE has clinical importance because it is responsible for immediate hypersensitivity. Studies of IgE expression in rats appear to contradict current models for immunoglobulin gene expression. To study rat IgE expression at the RNA and DNA levels, we have constructed a cDNA for part of the rat epsilon (ε) heavy chain that is expressed by a rat myeloma, IR162. The rat ε-chain clone was initially identified by an efficient selection scheme. DNA sequencing of the 580-bp cDNA revealed that it encoded 176 amino acids that were 50% homologous to the human εH chain. The sequence begins near the end of the CH2 domain and ends 31 amino acids into the CH4 domain. Cysteines important for the structure of the human IgE were conserved in the rat εH-chain. The identity of the cloned ε cDNA was confirmed by comparison with a portion of the constant region gene for mouse εH chain. The mouse and rat nucleotide sequences were 79% homologous.",

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journal = "DNA",

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T1 - A Cloned cDNA Probe for Rat Immunoglobulin Epsilon Heavy Chain

T2 - Construction, Identification, and DNA Sequence

AU - Kindsvogel, Wayne R.

AU - Reddy, E. Premkumar

AU - Moore, Joan M.

AU - Faust, Charles H.

PY - 1982

Y1 - 1982

N2 - IgE has clinical importance because it is responsible for immediate hypersensitivity. Studies of IgE expression in rats appear to contradict current models for immunoglobulin gene expression. To study rat IgE expression at the RNA and DNA levels, we have constructed a cDNA for part of the rat epsilon (ε) heavy chain that is expressed by a rat myeloma, IR162. The rat ε-chain clone was initially identified by an efficient selection scheme. DNA sequencing of the 580-bp cDNA revealed that it encoded 176 amino acids that were 50% homologous to the human εH chain. The sequence begins near the end of the CH2 domain and ends 31 amino acids into the CH4 domain. Cysteines important for the structure of the human IgE were conserved in the rat εH-chain. The identity of the cloned ε cDNA was confirmed by comparison with a portion of the constant region gene for mouse εH chain. The mouse and rat nucleotide sequences were 79% homologous.

AB - IgE has clinical importance because it is responsible for immediate hypersensitivity. Studies of IgE expression in rats appear to contradict current models for immunoglobulin gene expression. To study rat IgE expression at the RNA and DNA levels, we have constructed a cDNA for part of the rat epsilon (ε) heavy chain that is expressed by a rat myeloma, IR162. The rat ε-chain clone was initially identified by an efficient selection scheme. DNA sequencing of the 580-bp cDNA revealed that it encoded 176 amino acids that were 50% homologous to the human εH chain. The sequence begins near the end of the CH2 domain and ends 31 amino acids into the CH4 domain. Cysteines important for the structure of the human IgE were conserved in the rat εH-chain. The identity of the cloned ε cDNA was confirmed by comparison with a portion of the constant region gene for mouse εH chain. The mouse and rat nucleotide sequences were 79% homologous.

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A Cloned cDNA Probe for Rat Immunoglobulin Epsilon Heavy Chain: Construction, Identification, and DNA Sequence

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