A cellular resolution atlas of Broca's area

Irene Costantini; Leah Morgan; Jiarui Yang; Yael Balbastre; Divya Varadarajan; Luca Pesce; Marina Scardigli; Giacomo Mazzamuto; Vladislav Gavryusev; Filippo Maria Castelli; Matteo Roffilli; Ludovico Silvestri; Jessie Laffey; Sophia Raia; Merina Varghese; Bridget Wicinski; Shuaibin Chang; Ichun Anderson Chen; Hui Wang; Devani Cordero; Matthew Vera; Jackson Nolan; Kimberly Nestor; Jocelyn Mora; Juan Eugenio Iglesias; Erendira Garcia Pallares; Kathryn Evancic; Jean C. Augustinack; Morgan Fogarty; Adrian V. Dalca; Matthew P. Frosch; Caroline Magnain; Robert Frost; Andre van der Kouwe; Shih Chi Chen; David A. Boas; Francesco Saverio Pavone; Bruce Fischl; Patrick R. Hof

doi:10.1126/sciadv.adg3844

A cellular resolution atlas of Broca's area

Irene Costantini, Leah Morgan, Jiarui Yang, Yael Balbastre, Divya Varadarajan, Luca Pesce, Marina Scardigli, Giacomo Mazzamuto, Vladislav Gavryusev, Filippo Maria Castelli, Matteo Roffilli, Ludovico Silvestri, Jessie Laffey, Sophia Raia, Merina Varghese, Bridget Wicinski, Shuaibin Chang, Ichun Anderson Chen, Hui Wang, Devani CorderoMatthew Vera, Jackson Nolan, Kimberly Nestor, Jocelyn Mora, Juan Eugenio Iglesias, Erendira Garcia Pallares, Kathryn Evancic, Jean C. Augustinack, Morgan Fogarty, Adrian V. Dalca, Matthew P. Frosch, Caroline Magnain, Robert Frost, Andre van der Kouwe, Shih Chi Chen, David A. Boas, Francesco Saverio Pavone, Bruce Fischl, Patrick R. Hof

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custommade inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.

Original language	English
Article number	adg3844
Journal	Science advances
Volume	9
Issue number	41
DOIs	https://doi.org/10.1126/sciadv.adg3844
State	Published - Oct 2023

Access to Document

10.1126/sciadv.adg3844

Cite this

Costantini, I., Morgan, L., Yang, J., Balbastre, Y., Varadarajan, D., Pesce, L., Scardigli, M., Mazzamuto, G., Gavryusev, V., Castelli, F. M., Roffilli, M., Silvestri, L., Laffey, J., Raia, S., Varghese, M., Wicinski, B., Chang, S., Chen, I. A., Wang, H., ... Hof, P. R. (2023). A cellular resolution atlas of Broca's area. Science advances, 9(41), Article adg3844. https://doi.org/10.1126/sciadv.adg3844

@article{8128de0faefe406f890945fc2b08e321,

title = "A cellular resolution atlas of Broca's area",

abstract = "Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custommade inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.",

author = "Irene Costantini and Leah Morgan and Jiarui Yang and Yael Balbastre and Divya Varadarajan and Luca Pesce and Marina Scardigli and Giacomo Mazzamuto and Vladislav Gavryusev and Castelli, {Filippo Maria} and Matteo Roffilli and Ludovico Silvestri and Jessie Laffey and Sophia Raia and Merina Varghese and Bridget Wicinski and Shuaibin Chang and Chen, {Ichun Anderson} and Hui Wang and Devani Cordero and Matthew Vera and Jackson Nolan and Kimberly Nestor and Jocelyn Mora and Iglesias, {Juan Eugenio} and Pallares, {Erendira Garcia} and Kathryn Evancic and Augustinack, {Jean C.} and Morgan Fogarty and Dalca, {Adrian V.} and Frosch, {Matthew P.} and Caroline Magnain and Robert Frost and {van der Kouwe}, Andre and Chen, {Shih Chi} and Boas, {David A.} and Pavone, {Francesco Saverio} and Bruce Fischl and Hof, {Patrick R.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors.",

year = "2023",

month = oct,

doi = "10.1126/sciadv.adg3844",

language = "English",

volume = "9",

journal = "Science advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

number = "41",

}

Costantini, I, Morgan, L, Yang, J, Balbastre, Y, Varadarajan, D, Pesce, L, Scardigli, M, Mazzamuto, G, Gavryusev, V, Castelli, FM, Roffilli, M, Silvestri, L, Laffey, J, Raia, S, Varghese, M, Wicinski, B, Chang, S, Chen, IA, Wang, H, Cordero, D, Vera, M, Nolan, J, Nestor, K, Mora, J, Iglesias, JE, Pallares, EG, Evancic, K, Augustinack, JC, Fogarty, M, Dalca, AV, Frosch, MP, Magnain, C, Frost, R, van der Kouwe, A, Chen, SC, Boas, DA, Pavone, FS, Fischl, B & Hof, PR 2023, 'A cellular resolution atlas of Broca's area', Science advances, vol. 9, no. 41, adg3844. https://doi.org/10.1126/sciadv.adg3844

TY - JOUR

T1 - A cellular resolution atlas of Broca's area

AU - Costantini, Irene

AU - Morgan, Leah

AU - Yang, Jiarui

AU - Balbastre, Yael

AU - Varadarajan, Divya

AU - Pesce, Luca

AU - Scardigli, Marina

AU - Mazzamuto, Giacomo

AU - Gavryusev, Vladislav

AU - Castelli, Filippo Maria

AU - Roffilli, Matteo

AU - Silvestri, Ludovico

AU - Laffey, Jessie

AU - Raia, Sophia

AU - Varghese, Merina

AU - Wicinski, Bridget

AU - Chang, Shuaibin

AU - Chen, Ichun Anderson

AU - Wang, Hui

AU - Cordero, Devani

AU - Vera, Matthew

AU - Nolan, Jackson

AU - Nestor, Kimberly

AU - Mora, Jocelyn

AU - Iglesias, Juan Eugenio

AU - Pallares, Erendira Garcia

AU - Evancic, Kathryn

AU - Augustinack, Jean C.

AU - Fogarty, Morgan

AU - Dalca, Adrian V.

AU - Frosch, Matthew P.

AU - Magnain, Caroline

AU - Frost, Robert

AU - van der Kouwe, Andre

AU - Chen, Shih Chi

AU - Boas, David A.

AU - Pavone, Francesco Saverio

AU - Fischl, Bruce

AU - Hof, Patrick R.

PY - 2023/10

Y1 - 2023/10

N2 - Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custommade inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.

AB - Brain cells are arranged in laminar, nuclear, or columnar structures, spanning a range of scales. Here, we construct a reliable cell census in the frontal lobe of human cerebral cortex at micrometer resolution in a magnetic resonance imaging (MRI)-referenced system using innovative imaging and analysis methodologies. MRI establishes a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with a digital stereological approach on the 3D reconstruction at cellular resolution from a custommade inverted confocal light-sheet fluorescence microscope (LSFM). Mesoscale optical coherence tomography enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system. The outcome is an integrated high-resolution cellular census of Broca's area in a human postmortem specimen, within a whole-brain reference space atlas.

UR - http://www.scopus.com/inward/record.url?scp=85174751485&partnerID=8YFLogxK

U2 - 10.1126/sciadv.adg3844

DO - 10.1126/sciadv.adg3844

M3 - Article

C2 - 37824623

AN - SCOPUS:85174751485

SN - 2375-2548

VL - 9

JO - Science advances

JF - Science advances

IS - 41

M1 - adg3844

ER -

A cellular resolution atlas of Broca's area

Abstract

Access to Document

Fingerprint

Cite this