12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid lines

Manuel Patarroyo; Peter Biberfeld; Eva Klein; George Klein

doi:10.1016/0008-8749(81)90003-4

12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid lines

Manuel Patarroyo
, Peter Biberfeld
, Eva Klein
, George Klein

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The natural killer (NK) sensitivity of two Epstein-Barr virus (EBV)-carrying Burkitt lymphoma lines (Daudi and Raji) and one EBV-negative acute lymphocytic leukemia-derived T-cell line (Molt-4) increased when they were cultured for 24 hr in the presence of the phorbol ester TPA. In the EBV-carrying lines, this increase occurred independently of the entry of the cells into the viral cycle. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-treated Daudi and Molt-4 cells cross-competed for the effectors in the NK assay as indicated by the cold-target inhibition tests. TPA-treated cells showed an increased binding of human but not of mouse lymphocytes.

Original language	English
Pages (from-to)	237-248
Number of pages	12
Journal	Cellular Immunology
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(81)90003-4
State	Published - 15 Sep 1981
Externally published	Yes

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@article{7cc7414b14fb44278cc5bc3ea18b6380,

title = "12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid lines",

abstract = "The natural killer (NK) sensitivity of two Epstein-Barr virus (EBV)-carrying Burkitt lymphoma lines (Daudi and Raji) and one EBV-negative acute lymphocytic leukemia-derived T-cell line (Molt-4) increased when they were cultured for 24 hr in the presence of the phorbol ester TPA. In the EBV-carrying lines, this increase occurred independently of the entry of the cells into the viral cycle. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-treated Daudi and Molt-4 cells cross-competed for the effectors in the NK assay as indicated by the cold-target inhibition tests. TPA-treated cells showed an increased binding of human but not of mouse lymphocytes.",

author = "Manuel Patarroyo and Peter Biberfeld and Eva Klein and George Klein",

note = "Funding Information: This projecth as beens upportedb y Grant 2 ROl CA 14054-07Al (to G. Klein) and by Grant 1 ROl CA 23184-OlAl (to E. Klein) awarded by the National Cancer Institute, NIH, DHEW, and by the SwedishC ancer Society. We thank Dr. Mans Ullberg for helpful suggestionsc oncerningt he conjugate assay. We also thank Ms. Maria Karlsson and Mona Hedenskogf or valuable technical assistance. Manuel Patarroyo is a recipiento f a fellowshipf rom the National University of Colombia.",

year = "1981",

month = sep,

day = "15",

doi = "10.1016/0008-8749(81)90003-4",

language = "English",

volume = "63",

pages = "237--248",

journal = "Cellular Immunology",

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publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid lines

AU - Patarroyo, Manuel

AU - Biberfeld, Peter

AU - Klein, Eva

AU - Klein, George

N1 - Funding Information: This projecth as beens upportedb y Grant 2 ROl CA 14054-07Al (to G. Klein) and by Grant 1 ROl CA 23184-OlAl (to E. Klein) awarded by the National Cancer Institute, NIH, DHEW, and by the SwedishC ancer Society. We thank Dr. Mans Ullberg for helpful suggestionsc oncerningt he conjugate assay. We also thank Ms. Maria Karlsson and Mona Hedenskogf or valuable technical assistance. Manuel Patarroyo is a recipiento f a fellowshipf rom the National University of Colombia.

PY - 1981/9/15

Y1 - 1981/9/15

N2 - The natural killer (NK) sensitivity of two Epstein-Barr virus (EBV)-carrying Burkitt lymphoma lines (Daudi and Raji) and one EBV-negative acute lymphocytic leukemia-derived T-cell line (Molt-4) increased when they were cultured for 24 hr in the presence of the phorbol ester TPA. In the EBV-carrying lines, this increase occurred independently of the entry of the cells into the viral cycle. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-treated Daudi and Molt-4 cells cross-competed for the effectors in the NK assay as indicated by the cold-target inhibition tests. TPA-treated cells showed an increased binding of human but not of mouse lymphocytes.

AB - The natural killer (NK) sensitivity of two Epstein-Barr virus (EBV)-carrying Burkitt lymphoma lines (Daudi and Raji) and one EBV-negative acute lymphocytic leukemia-derived T-cell line (Molt-4) increased when they were cultured for 24 hr in the presence of the phorbol ester TPA. In the EBV-carrying lines, this increase occurred independently of the entry of the cells into the viral cycle. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-treated Daudi and Molt-4 cells cross-competed for the effectors in the NK assay as indicated by the cold-target inhibition tests. TPA-treated cells showed an increased binding of human but not of mouse lymphocytes.

UR - https://www.scopus.com/pages/publications/0019502467

U2 - 10.1016/0008-8749(81)90003-4

DO - 10.1016/0008-8749(81)90003-4

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AN - SCOPUS:0019502467

SN - 0008-8749

VL - 63

SP - 237

EP - 248

JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

12-O-tetradecanoylphorbol-13-acetate (TPA) treatment elevates the natural killer (NK) sensitivity of certain human lymphoid lines

Abstract

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