1, 25-dihydroxyvitamin D3 regulates proliferation of activated T-lymphocyte subsets

Toshimitsu Matsui; Yoshinobu Nakao; Tamio Koizumi; Toshitaro Nakagawa; Takuo Fujita

doi:10.1016/0024-3205(85)90630-7

1, 25-dihydroxyvitamin D₃ regulates proliferation of activated T-lymphocyte subsets

Toshimitsu Matsui
, Yoshinobu Nakao
, Tamio Koizumi
, Toshitaro Nakagawa
, Takuo Fujita

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The biologically active vitamin D metabolite, 1,25-(OH)₂D₃ suppressed phytohaemagglutinin (PHA)-induced lymphocyte proliferation dose-dependently (0.1 nM-100nM), and decreased the OKT4⁺/OKT8⁺ ratio and transferrin-receptor-positive (OKT9⁺) cells. A possible parallelism between expression of 1,25-(OH) ₂D₃ receptors and interleukin 2 (IL2)-receptors recognized by anti-Tac antibody was not confirmed in this study. However, the addition of exogenous IL2 abolished the inhibitory effects of 1,25-(OH) ₂D₃ on PHA-stimulated T-cell proliferation, and the decrease of OKT4⁺ and OKT9⁺ T-cell in this population. Among various vitamin D₃ analogues examined, 1,25-(OH) ₂D₃ was the most potent antiproliferative effect, followed in order by 1,24S-(OH) ₂D₃, 1α OH D₃, 25 OH D₃ and 24, 25-(OH) ₂D₃.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Life Sciences
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0024-3205(85)90630-7
State	Published - 8 Jul 1985
Externally published	Yes

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@article{3bbaae75e71d4ed5a7192de38736eb54,

title = "1, 25-dihydroxyvitamin D3 regulates proliferation of activated T-lymphocyte subsets",

abstract = "The biologically active vitamin D metabolite, 1,25-(OH)2D3 suppressed phytohaemagglutinin (PHA)-induced lymphocyte proliferation dose-dependently (0.1 nM-100nM), and decreased the OKT4+/OKT8+ ratio and transferrin-receptor-positive (OKT9+) cells. A possible parallelism between expression of 1,25-(OH) 2D3 receptors and interleukin 2 (IL2)-receptors recognized by anti-Tac antibody was not confirmed in this study. However, the addition of exogenous IL2 abolished the inhibitory effects of 1,25-(OH) 2D3 on PHA-stimulated T-cell proliferation, and the decrease of OKT4+ and OKT9+ T-cell in this population. Among various vitamin D3 analogues examined, 1,25-(OH) 2D3 was the most potent antiproliferative effect, followed in order by 1,24S-(OH) 2D3, 1α OH D3, 25 OH D3 and 24, 25-(OH) 2D3.",

author = "Toshimitsu Matsui and Yoshinobu Nakao and Tamio Koizumi and Toshitaro Nakagawa and Takuo Fujita",

year = "1985",

month = jul,

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doi = "10.1016/0024-3205(85)90630-7",

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pages = "95--101",

journal = "Life Sciences",

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publisher = "Elsevier Inc.",

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T1 - 1, 25-dihydroxyvitamin D3 regulates proliferation of activated T-lymphocyte subsets

AU - Matsui, Toshimitsu

AU - Nakao, Yoshinobu

AU - Koizumi, Tamio

AU - Nakagawa, Toshitaro

AU - Fujita, Takuo

PY - 1985/7/8

Y1 - 1985/7/8

N2 - The biologically active vitamin D metabolite, 1,25-(OH)2D3 suppressed phytohaemagglutinin (PHA)-induced lymphocyte proliferation dose-dependently (0.1 nM-100nM), and decreased the OKT4+/OKT8+ ratio and transferrin-receptor-positive (OKT9+) cells. A possible parallelism between expression of 1,25-(OH) 2D3 receptors and interleukin 2 (IL2)-receptors recognized by anti-Tac antibody was not confirmed in this study. However, the addition of exogenous IL2 abolished the inhibitory effects of 1,25-(OH) 2D3 on PHA-stimulated T-cell proliferation, and the decrease of OKT4+ and OKT9+ T-cell in this population. Among various vitamin D3 analogues examined, 1,25-(OH) 2D3 was the most potent antiproliferative effect, followed in order by 1,24S-(OH) 2D3, 1α OH D3, 25 OH D3 and 24, 25-(OH) 2D3.

AB - The biologically active vitamin D metabolite, 1,25-(OH)2D3 suppressed phytohaemagglutinin (PHA)-induced lymphocyte proliferation dose-dependently (0.1 nM-100nM), and decreased the OKT4+/OKT8+ ratio and transferrin-receptor-positive (OKT9+) cells. A possible parallelism between expression of 1,25-(OH) 2D3 receptors and interleukin 2 (IL2)-receptors recognized by anti-Tac antibody was not confirmed in this study. However, the addition of exogenous IL2 abolished the inhibitory effects of 1,25-(OH) 2D3 on PHA-stimulated T-cell proliferation, and the decrease of OKT4+ and OKT9+ T-cell in this population. Among various vitamin D3 analogues examined, 1,25-(OH) 2D3 was the most potent antiproliferative effect, followed in order by 1,24S-(OH) 2D3, 1α OH D3, 25 OH D3 and 24, 25-(OH) 2D3.

UR - https://www.scopus.com/pages/publications/0021888713

U2 - 10.1016/0024-3205(85)90630-7

DO - 10.1016/0024-3205(85)90630-7

M3 - Article

C2 - 2989641

AN - SCOPUS:0021888713

SN - 0024-3205

VL - 37

SP - 95

EP - 101

JO - Life Sciences

JF - Life Sciences

IS - 1

ER -

1, 25-dihydroxyvitamin D3 regulates proliferation of activated T-lymphocyte subsets

Abstract

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1, 25-dihydroxyvitamin D₃ regulates proliferation of activated T-lymphocyte subsets