结核分枝杆菌EF4蛋白在应激条件下的功能研究

Objective: To investigate the function of Mycobacterium tuberculosis EF4 protein in response to stress. Methods: The plasmid pSMT-EF4 encoding Mycobacterium tuberculosis EF4 protein was transformed into Escherichia coli (E.coli) BL21 to make it express EF4 protein. The successfully transformed E. coli BL21 strains were cultured under different stress conditions and the growth curves were recorded to study the function of EF4 protein in response to stress. Results: In three media containing ampicillin, G418 and streptomycin respectively, the growth of E. coli BL21 expressing Mycobacterium tuberculosis EF4 protein was better than that of untransformed E. coli BL21, and significant differences were observed after culturing for 6 h, 9 h and 2 h, respectively. No significant difference in bacterial growth was found between experimental and control groups cultured under the condition of H₂O₂-containing medium, pH3, 20℃ or 45℃. Conclusion: E. coli BL21 overexpressing Mycobacterium tuberculosis EF4 protein showed certain resistance to antibiotics. Mycobacterium tuberculosis EF4 protein was not directly involved in responses to oxidative, acidic and temperature stresses.