灵芝中 C₂H₂ 锌指蛋白家族全基因组鉴定及表达分析

Objective Based on the genome and transcriptome data of Ganoderma lucidum, this study aims to explore the members of the C₂H₂ zinc finger proteins (C₂H₂-ZFPs) gene family in G.lucidum, and analyze their expression patterns in mycelium induced by methyl jasmonate (MeJA), in order to discover C₂H₂-ZFPs that play a role in the growth and development, stress response, and secondary metabolism of G.lucidum. Methods Genome-wide identification of C₂H₂ transcription factors in G.lucidum was carried out by Hidden Markov Model search and conserved structural domain analysis, physicochemical properties of C₂H₂-ZFPs were analyzed by Peptides software package, phylogenetic tree of C₂H₂ zinc finger proteins was constructed by using IQtree, and the conserved motifs and gene structures were analyzed by using MEME and GSDS₂.0 online tools, and the relative expression of C₂H₂ gene in mycelium after MeJA induction was determined by qRT-PCR. Results G.lucidum contains 20 C₂H₂-type gene family members (GlC₂H₂.01—GlC₂H₂.20), which can be categorized into five subgroups and distributed on 10 chromosomes of G.lucidum. Phylogenetic and conserved motifs revealed that C₂H₂ transcription factors in G.lucidum are evolutionarily conserved and contain at least one zf-C₂H₂, and promoter region prediction analysis identified multiple classes of hormones and adversity-responsive cis-acting elements. Transcriptome analysis results showed that C₂H₂ gene was most highly expressed in the primary base stage of G.lucidum, and was hardly expressed in sporopollen. The expression of GlC₂H₂.11 and GlC₂H₂.20 in the mycelium was significantly decreased after 9 h of MeJA induction, and the other GlC₂H₂ showed a decreasing trend from 3 to 9 h of treatment, and then recovered to the normal expression level after 12 h. Conclusion The systematic identification of C₂H₂ transcription factor gene family from the whole genome of G. lucidum provides a theoretical reference for further research on the biological functions of C₂H₂ transcription factors in regulating the development and secondary metabolism of G. lucidum.