Abstract
Objective: In order to provide a basis for EuTCPs gene function research, Eucommia ulmoides TCP (Teosinte branched 1/Cycloidea/Proliferating) gene family was screened and analyzed in this study. Methods: Based on E. ulmoides genome database, bioinformatics methods was used to systematically analyze the physical and chemical properties, phylogenetic evolution, gene structure, promoter cis-acting element and their expression in leaf development and gum formation of LBD gene family. Results: In this study, a total of 14 EuTCPs were identified from E. ulmoides genome, EuTCPs encodes 139-492 amino acids, and theoretical isoelectric points distribution were 5.54-9.72, molecular weight between 18 880 to 53 620 by physicochemical analysis, all of EuTCPs proteins were hydrophilic proteins and expressed in nucleus. EuTCP gene family was divided into three subfamilies: PCF, CIN and CYC/TB1, which contain five, six and three EuTCPs proteins respectively. Expression pattern analysis showed that EuTCPs played different roles in leaf development of E. ulmoides and positively regulated the formation of gutta-percha. Conclusion: In this study, the members of the TCP family were comprehensively identified and bioinformatics analysis from the genomic level of E. ulmoides, which provides basic data for further functional research of EuTCP genes.
| Translated title of the contribution | Identification and bioinformatics analysis of TCP transcription factors in Eucommia ulmoides |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 3755-3765 |
| Number of pages | 11 |
| Journal | Chinese Traditional and Herbal Drugs |
| Volume | 53 |
| Issue number | 12 |
| DOIs | |
| State | Published - 28 Jun 2022 |
| Externally published | Yes |
Keywords
- Eucommia ulmoides Oliver
- Expression analysis
- Leaf development
- Phylogeny evolution
- TCP gene family