多模态斖斠斏观察电针治疗大鼠脑缺血再灌注后脑白质损伤

Objective To observe electroacupuncture for treating white matter injury after cerebral ischemia reperfusion based on multimodal MRI. Methods Totally 35 rats were divided into sham operation (Sham) group (n=5), cerebral ischemia reperfusion injury (CIRI) group (n = 15), or electroacupuncture group (n = 15) and underwent relative managements. Cranial T2WI, diffusion weighted imaging and diffusion tensor imaging were acquired 24 h, 72 h and 5 d after managements, fraction anisotropy (FA) and Colored FA maps were obtained. The rats were then sacrificed, brain tissue were harvested and stained with mouse anti-myelin basic protein (MBP)/rabbit anti-neurofilament protein 200 (NF200) and mouse anti-proliferating cell nuclear antigen (PCNA)/rabbit anti-neuroglial antigen 2 (NG2) immunofluorescence double-label staining, and the effect of electroacupuncture on white matter injury and the proliferation of endogenous oligodendrocyte progenitor cells in the brain tissue of CIRI rats were observed. Results The volume of cerebral infarction in electroacupuncture group was significantly smaller than that in CIRI group 24 h, 72 h and 5 d after managements (all P<0.05). The relative apparent diffusion coefficient (rADC) and relative FA (rFA) of ischemic striatum and corpus callosum areas in CIRI group and electroacupuncture group were all lower than those in Sham group (all P<0.05), while of the striatum and corpus callosum areas in electroacupuncture group were all higher than those in CIRI group (all P<0.05). Five days after managements, the fluorescence intensity (FI) of MBP, NF200 of the ischemic striatum and corpus callosum, the number of cells with positive of PCNA and NG2 in the striatum in CIRI and electroacupuncture group were all lower than those in Sham group (all P<0.05). FIMBP, FINF2OO of the ischemic striatum and corpus callosum, the number of cells with positive of PCNA and NG2 in the striatum of rats in electroacupuncture group were all higher than those in CIRI group (all P<0.05). Conclusion Electroacupuncture could reduce white matter injury in CIRI rats and promote generation of PCNA and NG2.